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mt2 antibodies  (Alomone Labs)


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    Alomone Labs mt2 antibodies
    Mt2 Antibodies, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+mt2+antibodies/Anti-Melatonin+Receptor+1B+Antibody/pm41366729-81-10-13
    Average 93 stars, based on 8 article reviews
    mt2 antibodies - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Immunofluorescence:

    Article Title: Molecular Characterization and Pharmacology of Melatonin Receptors in Animals
    Article Snippet: Knock-out of MT2 in this cell line abolished the signal in immunofluorescence experiments and in the proximity ligation assay aiming to detect MT1/MT2 heteromers in combination with the anti-MT1 antibody from Alomone. .. Anti-MT2 antibodies from Alomone showed a similar immunoreactivity immunofluorescence experiments in 661W wild-type cells [155] and detected neurons in the periaqueductal gray in mice [156]. ..



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    Image Search Results


    Expression of MT1 and MT2 genes in rat TG, SPG and hypothalamus. ( A ) Comparison of MT1 and MT2 mRNA levels in the TG of male and female rats shows that MT1 expression is significantly higher than MT2. ( B ) In both the hypothalamus (HT) and SPG of male rats, MT1 mRNA levels are also significantly higher than MT2. Data are presented as mean ± SEM, statistical significance indicate as *** p < 0.001, ** p < 0.01, * p < 0.05, and n = 6

    Journal: The Journal of Headache and Pain

    Article Title: Expression of melatonin receptors in trigeminal and sphenopalatine ganglia: potential targets for primary headache disorders

    doi: 10.1186/s10194-025-02215-9

    Figure Lengend Snippet: Expression of MT1 and MT2 genes in rat TG, SPG and hypothalamus. ( A ) Comparison of MT1 and MT2 mRNA levels in the TG of male and female rats shows that MT1 expression is significantly higher than MT2. ( B ) In both the hypothalamus (HT) and SPG of male rats, MT1 mRNA levels are also significantly higher than MT2. Data are presented as mean ± SEM, statistical significance indicate as *** p < 0.001, ** p < 0.01, * p < 0.05, and n = 6

    Article Snippet: MT2 (AMR-032) , 1:200 , Rabbit , Alomone Labs, Jerusalem, Israel , AB_2340995.

    Techniques: Expressing, Comparison

    MT1 and MT2 immunoreactivity in TG. ( A ) positive MT1 immunoreactivity was observed in the cytoplasm and nuclei of TG neurons (thick arrows) and in SGCs (arrowheads). ( B ) MT2 immunoreactivity was observed predominantly in the cytoplasm of neurons (thick arrows), in some cases in their nuclei (thick arrows), and in Aδ-fibers (thin arrows). Negative cells are indicated by asterisks in both ( A ) and ( B ). Blue color represents nuclei staining with DAPI. ( C ) Bar graphs show the number of MT1 and MT2 immunoreactive TG neurons in male and female rats. There was no significant difference in MT1 or MT2 protein expression between sexes. However, the number of MT1-immunoreactive cells was approximately twofold higher than that of MT2 in both males and females. Data are presented as the mean ± SEM, n = 6 and * p < 0.05

    Journal: The Journal of Headache and Pain

    Article Title: Expression of melatonin receptors in trigeminal and sphenopalatine ganglia: potential targets for primary headache disorders

    doi: 10.1186/s10194-025-02215-9

    Figure Lengend Snippet: MT1 and MT2 immunoreactivity in TG. ( A ) positive MT1 immunoreactivity was observed in the cytoplasm and nuclei of TG neurons (thick arrows) and in SGCs (arrowheads). ( B ) MT2 immunoreactivity was observed predominantly in the cytoplasm of neurons (thick arrows), in some cases in their nuclei (thick arrows), and in Aδ-fibers (thin arrows). Negative cells are indicated by asterisks in both ( A ) and ( B ). Blue color represents nuclei staining with DAPI. ( C ) Bar graphs show the number of MT1 and MT2 immunoreactive TG neurons in male and female rats. There was no significant difference in MT1 or MT2 protein expression between sexes. However, the number of MT1-immunoreactive cells was approximately twofold higher than that of MT2 in both males and females. Data are presented as the mean ± SEM, n = 6 and * p < 0.05

    Article Snippet: MT2 (AMR-032) , 1:200 , Rabbit , Alomone Labs, Jerusalem, Israel , AB_2340995.

    Techniques: Staining, Expressing

    Double immunohistochemistry of MT2 with CGRP or RAMP1 in TG. ( A and D ) MT2 immunoreactivity (green fluorescence) was observed in cytoplasm of TG neurons (thick arrows), and in Aδ- fibers (arrowheads). ( B ) CGRP (red fluorescence) was expressed in small to medium-sized TG neurons (thick arrows) as well as in C-fibers (arrowhead). ( C ) MT2 co-localized with CGRP (yellow-orange) in the cytoplasm of small to medium-sized neurons (thick arrows). ( E ) RAMP1 (red fluorescence) was expressed in the cytoplasm of medium to large- sized TG neurons (thick arrow) and in Aδ-fibers (arrowhead). ( F ) The merged imaged showed that MT2 was co-localized (yellow) with RAMP1 in the cytoplasm of medium to large-sized neurons (thick arrow) as well as in the Aδ- fibers (arrowhead)

    Journal: The Journal of Headache and Pain

    Article Title: Expression of melatonin receptors in trigeminal and sphenopalatine ganglia: potential targets for primary headache disorders

    doi: 10.1186/s10194-025-02215-9

    Figure Lengend Snippet: Double immunohistochemistry of MT2 with CGRP or RAMP1 in TG. ( A and D ) MT2 immunoreactivity (green fluorescence) was observed in cytoplasm of TG neurons (thick arrows), and in Aδ- fibers (arrowheads). ( B ) CGRP (red fluorescence) was expressed in small to medium-sized TG neurons (thick arrows) as well as in C-fibers (arrowhead). ( C ) MT2 co-localized with CGRP (yellow-orange) in the cytoplasm of small to medium-sized neurons (thick arrows). ( E ) RAMP1 (red fluorescence) was expressed in the cytoplasm of medium to large- sized TG neurons (thick arrow) and in Aδ-fibers (arrowhead). ( F ) The merged imaged showed that MT2 was co-localized (yellow) with RAMP1 in the cytoplasm of medium to large-sized neurons (thick arrow) as well as in the Aδ- fibers (arrowhead)

    Article Snippet: MT2 (AMR-032) , 1:200 , Rabbit , Alomone Labs, Jerusalem, Israel , AB_2340995.

    Techniques: Immunohistochemistry, Fluorescence

    Double immunohistochemistry of MT2 and CASPR in trigeminal Aδ-fibers. ( A ) MT2 receptors (green) were found in the Aδ-fibers. ( B ) CASPR immunoreactivity (red) was used to label the paranodal regions of nodes of Ranvier. ( C ) MT2 and CASPR were co-expressed in Aδ-fibers. The blue color represents nuclei staining with DAPI

    Journal: The Journal of Headache and Pain

    Article Title: Expression of melatonin receptors in trigeminal and sphenopalatine ganglia: potential targets for primary headache disorders

    doi: 10.1186/s10194-025-02215-9

    Figure Lengend Snippet: Double immunohistochemistry of MT2 and CASPR in trigeminal Aδ-fibers. ( A ) MT2 receptors (green) were found in the Aδ-fibers. ( B ) CASPR immunoreactivity (red) was used to label the paranodal regions of nodes of Ranvier. ( C ) MT2 and CASPR were co-expressed in Aδ-fibers. The blue color represents nuclei staining with DAPI

    Article Snippet: MT2 (AMR-032) , 1:200 , Rabbit , Alomone Labs, Jerusalem, Israel , AB_2340995.

    Techniques: Immunohistochemistry, Staining

    Double immunohistochemistry of MT2 with CASPR and CGRP in rat dura mater. ( A and D ) Thin MT2-immunoreactive axons (green, arrows) were observed in the rat dura mater. ( B ) These fibers were identified as Aδ-fibers based on CASPR immunoreactivity (red) showing the characteristic bowtie-shaped paranodal pattern (arrowheads). ( A - C ) Fiber bundles were often seen flanking dural blood vessels (asterisks). ( C ) MT2 and CASPR were observed to be co-expressed in the same Aδ-fibers. ( E ) CGRP immunoreactivity (red, arrowheads) was detected in c-fibers throughout the rat dura mater in a granular, pearl-like pattern. ( F ) Merged images show intertwined C- and Aδ-fibers, expressing CGRP and MT2 respectively, projecting throughout the dura mater

    Journal: The Journal of Headache and Pain

    Article Title: Expression of melatonin receptors in trigeminal and sphenopalatine ganglia: potential targets for primary headache disorders

    doi: 10.1186/s10194-025-02215-9

    Figure Lengend Snippet: Double immunohistochemistry of MT2 with CASPR and CGRP in rat dura mater. ( A and D ) Thin MT2-immunoreactive axons (green, arrows) were observed in the rat dura mater. ( B ) These fibers were identified as Aδ-fibers based on CASPR immunoreactivity (red) showing the characteristic bowtie-shaped paranodal pattern (arrowheads). ( A - C ) Fiber bundles were often seen flanking dural blood vessels (asterisks). ( C ) MT2 and CASPR were observed to be co-expressed in the same Aδ-fibers. ( E ) CGRP immunoreactivity (red, arrowheads) was detected in c-fibers throughout the rat dura mater in a granular, pearl-like pattern. ( F ) Merged images show intertwined C- and Aδ-fibers, expressing CGRP and MT2 respectively, projecting throughout the dura mater

    Article Snippet: MT2 (AMR-032) , 1:200 , Rabbit , Alomone Labs, Jerusalem, Israel , AB_2340995.

    Techniques: Immunohistochemistry, Expressing

    Localization of MT2 immunoreactivity in the SPG and its co-localization with VIP and PACAP. ( A , D and G ) MT2 immunoreactivity (green) was observed in the cytoplasm of neurons (thick arrows), and in fibers (arrowhead). ( B and E ) VIP immunoreactivity (red) was detected in the cytoplasm of many medium-sized neurons (thick arrows) and in a few fibers (arrowheads). ( C and F ) MT2 was co-localized with VIP in the cytoplasm of medium sized neurons (yellow-orange, thick arrows). D , E and F are higher-magnification views of images A , B and C , respectively. ( H ) PACAP immunoreactivity (red) was found in the cytoplasm of SPG neurons (thick arrows). ( I ) Double staining of MT2 and PACAP showed that MT2 was co-localized with PACAP in the cytoplasm of SPG neurons (yellow-orange, thick arrow)

    Journal: The Journal of Headache and Pain

    Article Title: Expression of melatonin receptors in trigeminal and sphenopalatine ganglia: potential targets for primary headache disorders

    doi: 10.1186/s10194-025-02215-9

    Figure Lengend Snippet: Localization of MT2 immunoreactivity in the SPG and its co-localization with VIP and PACAP. ( A , D and G ) MT2 immunoreactivity (green) was observed in the cytoplasm of neurons (thick arrows), and in fibers (arrowhead). ( B and E ) VIP immunoreactivity (red) was detected in the cytoplasm of many medium-sized neurons (thick arrows) and in a few fibers (arrowheads). ( C and F ) MT2 was co-localized with VIP in the cytoplasm of medium sized neurons (yellow-orange, thick arrows). D , E and F are higher-magnification views of images A , B and C , respectively. ( H ) PACAP immunoreactivity (red) was found in the cytoplasm of SPG neurons (thick arrows). ( I ) Double staining of MT2 and PACAP showed that MT2 was co-localized with PACAP in the cytoplasm of SPG neurons (yellow-orange, thick arrow)

    Article Snippet: MT2 (AMR-032) , 1:200 , Rabbit , Alomone Labs, Jerusalem, Israel , AB_2340995.

    Techniques: Double Staining

    MT1 and MT2 immunoreactivity in the DRG. ( A ) MT1 expression in DRG was observed in both the cytoplasm and nuclei of neurons (thick arrow) and in SGCs (arrowheads). ( B ) MT2 immunoreactivity was localized in the cytoplasm of neurons and in some cases in their nuclei (thick arrow), and in the Aδ-fibers (thin arrows). The blue color represents nuclei staining with DAPI

    Journal: The Journal of Headache and Pain

    Article Title: Expression of melatonin receptors in trigeminal and sphenopalatine ganglia: potential targets for primary headache disorders

    doi: 10.1186/s10194-025-02215-9

    Figure Lengend Snippet: MT1 and MT2 immunoreactivity in the DRG. ( A ) MT1 expression in DRG was observed in both the cytoplasm and nuclei of neurons (thick arrow) and in SGCs (arrowheads). ( B ) MT2 immunoreactivity was localized in the cytoplasm of neurons and in some cases in their nuclei (thick arrow), and in the Aδ-fibers (thin arrows). The blue color represents nuclei staining with DAPI

    Article Snippet: MT2 (AMR-032) , 1:200 , Rabbit , Alomone Labs, Jerusalem, Israel , AB_2340995.

    Techniques: Expressing, Staining